healthy offspring from freeze dried mouse spermatozoa

Space mice and normalcy of spermatozoa DNA preserved

Mouse sperm stored on International Space Station

 · This September 11 2020 image courtesy of Teruhiko Wakayama University of Yamanashi shows healthy offspring and next generation of mice derived from space preserved spermatozoa.

Tolerance of the freeze-dried mouse sperm nucleus to

When mouse spermatozoa were freeze-dried (FD) similar to the anhydrobiosis of Tardigrades all spermatozoa were ostensibly dead after rehydration. However offspring were obtained from recovered FD sperm nuclei even after repeated treatment with conditions from liquid nitrogen to room temperature.

Mouse sperm stored on ISS produces healthy young Space

 · Healthy offspring and next generation of mice derived from space preserved spermatozoa on September 11 2020 AFP The sperm was stored in the International Space Station in freeze-dried form for almost six years

Freeze-dried sperm stored on ISS breeds healthy mice on Earth

 · Fast forward a mouse baby shower or two and the team has proceeded to breed litters of healthy "space pups." In order to better determine the long-term effects of space radiation on mammalian sperm the researchers collected samples from healthy mice on Earth which were then freeze dried to remove any water.

Production of Live Offspring from Hamster Oocytes Injected

Freeze-drying storage of mouse spermatozoa is now becoming a new preservation method for the maintenance of genetic resources. However the freeze-drying method

Freeze‐dried spermatozoa A future tool

Freeze-dried sperm storage times can be very long ranging from days to years. Kaneko and Serikawa (2012a) found no significant differ-ences in the rate of development for the offspring of embryos produced from freeze-dried mouse spermatozoa stored for 3 years when com-pared with fresh or freeze-dried spermatozoa stored for a short term.

Production of Live Offspring from Hamster Oocytes Injected

Freeze-drying storage of mouse spermatozoa is now becoming a new preservation method for the maintenance of genetic resources. However the freeze-drying method

Freeze‐dried spermatozoa A future tool Olaciregui

 · Freeze-dried sperm storage times can be very long ranging from days to years. Kaneko and Serikawa found no significant differences in the rate of development for the offspring of embryos produced from freeze-dried mouse spermatozoa stored for 3 years when compared with fresh or freeze-dried spermatozoa stored for a short term.

Freeze-dried mouse sperm remains viable after being in

 · But it could soon become a reality after a study found freeze-dried mouse sperm remained viable after being on the International Space Station (ISS) for almost six years. Not only did radiation not affect the sperm s DNA or its ability to produce healthy space pups scientists estimate it could actually be preserved in space for more

After 9 months in space mouse sperm yield healthy mice

 · healthy offspring resulted. The baby mice grew into "Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months " PNAS (2017).

Freeze-dried mouse sperm remains viable after being in

 · Study found freeze-dried mouse sperm still viable after nearly six years in space They found it did not affect sperm DNA or ability to produce healthy space pups We obtained many healthy "space pup" offspring from space-preserved spermatozoa with the same success rate as the ground controls the authors said.

Mouse sperm stored on Space Station produces healthy

 · Space pups are here Mouse sperm stored on Space Station produces healthy offspring The sperm was stored in the International Space Station in freeze-dried form. Once brought back to Earth and rehydrated it resulted in the birth of 168 young free of genetic defects.

Long-term preservation of freeze-dried mouse spermatozoa

 · In summary we reported here the successful long-term preservation of freeze-dried mouse spermatozoa at 4 °C and offspring with fertility was obtained from oocytes microinjected with these spermatozoa.Fig. 1 shows a flowchart from sperm preservation by freeze-drying to production of offspring based on our present and previous studies ccessful sperm freeze-drying in C57BL/6J as well as

Freeze‐dried spermatozoa A future tool Olaciregui

 · Freeze-dried sperm storage times can be very long ranging from days to years. Kaneko and Serikawa found no significant differences in the rate of development for the offspring of embryos produced from freeze-dried mouse spermatozoa stored for 3 years when compared with fresh or freeze-dried spermatozoa stored for a short term.

Healthy offspring from freeze-dried mouse spermatozoa

 · Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months. Sayaka Wakayama Yuko Kamada Kaori Yamanaka Takashi Kohda Hiromi Suzuki Toru Shimazu Motoki N Tada Ikuko Osada Aiko Nagamatsu Satoshi Kamimura Hiroaki Nagatomo Eiji Mizutani Fumitoshi Ishino Sachiko Yano Teruhiko Wakayama

Space radiobiology needs realistic hypotheses and

1 Wakayama S et al. (2017) Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months. Proc Natl Acad Sci USA 114 5988–5993. 2 Cucinotta FA (2015) Review of NASA approach to space radiation risk assessments

Long-term preservation of freeze-dried mouse spermatozoa

 · In summary we reported here the successful long-term preservation of freeze-dried mouse spermatozoa at 4 °C and offspring with fertility was obtained from oocytes microinjected with these spermatozoa.Fig. 1 shows a flowchart from sperm preservation by freeze-drying to production of offspring based on our present and previous studies ccessful sperm freeze-drying in C57BL/6J as well as

Advance Publication by J-STAGE Journal of Reproduction

44 spermatozoa 1 . Besides work on the mouse model the birth of a healthy offspring using injected 45 epididymal freeze-dried spermatozoa has previously only been achieved in rat and hamster models 2–4 . In 46 other species such as rabbit 5 and horse 6 where offspring were obtained ejaculated spermatozoa were 47 used.

Tolerance of the freeze-dried mouse sperm nucleus to

When mouse spermatozoa were freeze-dried (FD) similar to the anhydrobiosis of Tardigrades all spermatozoa were ostensibly dead after rehydration. However offspring were obtained from recovered FD sperm nuclei even after repeated treatment with conditions from liquid nitrogen to room temperature.

Healthy offspring from freeze-dried mouse spermatozoa

When spermatozoa are freeze-dried or evaporatively dried none of the sperm survive however mouse spermatozoa can maintain the ability to generate offspring when added to water and microinjected into fresh oocytes (31 32) and this may possible with human spermatozoa in the future (33 34).

Freeze-Dried Mouse Sperm Survived 6 Years in Space Used

 · The researchers also hit mouse freeze-dried sperm with X-rays on Earth and found that sperm exposed to such radiation could still produce healthy pups. The researchers noted that although there are differences in the DNA damage caused by X-rays versus space radiation they estimate that freeze-dried mouse sperm can be preserved on the ISS for over 200 years before becoming unviable.